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          RabbitAnti-5HT2A Receptor antibody (bs-1056R)
          ~~~促銷,代碼SSY221101~~~
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          產品編號 bs-1056R
          英文名稱 5HT2A Receptor
          中文名稱 5-羥色胺受體2A抗體
          別    名 5HT2A_HUMAN; 5-hydroxytryptamine receptor 2A; HTR2A; HTR2; 5-HT-2; 5-HT-2A; Serotonin receptor 2A;  
          Specific References  (4)     |     bs-1056R has been referenced in 4 publications.
          [IF=3.395] Xiao cui Yuan. et al. Spinal 5-HT2A Receptor is Involved in Electroacupuncture Inhibition of Chronic Pain :. Mol Pain. 0;():  WB ;  Mouse.  
          [IF=2.72] Lan Yao. et al. Armillaria mellea fermentation liquor ameliorates p-chlorophenylalanine-induced insomnia associated with the modulation of serotonergic system and gut microbiota in rats. 2022 Jan 05  WB,IHC ;  Rat.  
          [IF=14.971] Kyritsi K et al. Modulation of the TPH 1/MAO‐A/5 HT/5 HTR 2A/2B/2C Axis Regulates Biliary Proliferation and Liver Fibrosis During Cholestasis. Hepatology.2019 Jul 25.  ICF&IHC ;  Rat&Mouse.  
          [IF=2.59] Sun, Tao, et al. "Antihypertensive effect of formononetin through regulating the expressions of eNOS, 5-HT< sub> 2A/1B receptors and ??< sub> 1-adrenoceptors in spontaneously hypertensive rat arteries."European journal of pharmacology (2013)..  Rat.  
          研究領域 神經生物學  通道蛋白  
          抗體來源 Rabbit
          克隆類型 Polyclonal
          交叉反應 Human, Mouse, Rat, 
          產品應用 WB=1:500-2000 ELISA=1:5000-10000 IHC-F=1:100-500 IF=1:200-800 (石蠟切片需做抗原修復)
          not yet tested in other applications.
          optimal dilutions/concentrations should be determined by the end user.
          理論分子量 52kDa
          細胞定位 細胞膜 
          性    狀 Liquid
          濃    度 1mg/ml
          免 疫 原 KLH conjugated synthetic peptide derived from human 5HT2A Receptor: 65-160/471 <Cytoplasmic>
          亞    型 IgG
          純化方法 affinity purified by Protein A
          緩 沖 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
          保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
          注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
          PubMed PubMed
          產品介紹 5HT2A receptor is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. 5HT2A receptor belongs to the G-protein coupled receptor 1 family and mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. This receptor is involved in tracheal smooth muscle contraction, bronchoconstriction, and control of aldosterone production. 5HT2A receptor is an integral membrane protein which localizes to the post-synaptic thickening of axo-dendritic synapses. 5HT2A receptor protein contains a PDZ domain-binding motif which is involved in the interaction with INADL, CASK, APBA1, DLG1 and DLG4.

          Function:
          This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. This receptor is involved in tracheal smooth muscle contraction, bronchoconstriction, and control of aldosterone production.

          Subunit:
          Interacts with MPDZ and INADL. May interact with MPP3, PRDX6, DLG4, DLG1, CASK, APBA1 and MAGI2.

          Subcellular Location:
          Cell membrane; Multi-pass membrane protein. Note=Localizes to the postsynaptic thickening of axo-dendritic synapses.

          Similarity:
          Belongs to the G-protein coupled receptor 1 family.

          SWISS:
          P28223

          Gene ID:
          3356

          Database links:

          Entrez Gene: 3356 Human

          Entrez Gene: 15558 Mouse

          Entrez Gene: 29595 Rat

          Omim: 182135 Human

          SwissProt: P28223 Human

          SwissProt: P35363 Mouse

          SwissProt: P14842 Rat

          Unigene: 654586 Human

          Unigene: 72630 Human

          Unigene: 214351 Mouse

          Unigene: 10294 Rat

          Unigene: 146756 Rat



          產品圖片
          Sample:
          Lane 1: Cerebrum (Mouse) Lysate at 40 ug
          Lane 2: Cerebrum (Rat) Lysate at 40 ug
          Primary: Anti-5HT2A Receptor (bs-1056R) at 1/1000 dilution
          Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
          Predicted band size: 53 kD
          Observed band size: 50 kD
          Sample:
          U251(Human) Cell Lysate at 30 ug
          BV2(Mouse) Cell Lysate at 30 ug
          Primary: Anti-5HT2A Receptor (bs-1056R) at 1/300 dilution
          Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
          Predicted band size: 52 kD
          Observed band size: 52 kD
          Tissue/cell: rat lung tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
          Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
          Incubation: Anti-5HT2A Receptor Polyclonal Antibody, Unconjugated(bs-1056R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
          Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5HT2A Receptor) Polyclonal Antibody, Unconjugated (bs-1056R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G- cy3) for 90 minutes, and DAPI for nuclei staining.
          Blank control: U-2OS.
          Primary Antibody (green line): Rabbit Anti-5HT2A Receptor antibody (bs-1056R)
          Dilution: 3μg /10^6 cells;
          Isotype Control Antibody (orange line): Rabbit IgG .
          Secondary Antibody : Goat anti-rabbit IgG-AF647
          Dilution: 3μg /test.
          Protocol
          The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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