<samp id="p54jh"></samp>
  • <td id="p54jh"></td>

    <acronym id="p54jh"><strong id="p54jh"></strong></acronym>
    <track id="p54jh"></track>
    1. 客服熱線:400-901-9800          客服QQ:4009019800          技術答疑   技術支持   質量反饋   人才招聘   關于我們   聯系我們
      首頁 > 產品中心 > 一抗 > 產品信息
      Rabbit Anti-TrkB antibody (bs-0175R)
      說明書: 50ul  100ul  200ul

      產品編號 bs-0175R
      英文名稱 TrkB
      中文名稱 酪氨酸激酶B抗體
      別    名 TrkB; NTRK2; Tyrosine Receptor Kinase B; Tyrosine kinase,Pyk2; BDNF tropomyosine receptor kinase B; BDNF/NT 3 growth factors receptor; BDNF/NT-3 growth factors receptor; Brain derived neurotrophic factor receptor; GP145 TrkB; GP145-TrkB; GP145-TrkB/GP95-TrkB; GP95 TrkB; Neurotrophic tyrosine kinase receptor type 2; Neurotrophin receptor tyrosine kinase type 2; NTRK 2; Ntrk2; NTRK2_HUMAN; Obesity, hyperphagia, and developmental delay, included; RATTRKB1; Tkrb; Trk B; Trk-B; TRKB; TrkB tyrosine kinase; TRKB1; tyrosine kinase receptor B; Tyrosine receptor kinase B.  
      Specific References  (3)     |     bs-0175R has been referenced in 3 publications.
      [IF=5.151] Li Q et al. Cerebrosides from Sea Cucumber Improved Aβ1‐42 Induced Cognitive Deficiency in a Rat Model of Alzheimer's Disease.(2018) Mol Nutr Food Res.   WB ;  Rat .  
      [IF=2.17] Zhu, Xiaoxia, et al. "Xiao Yao San improves depressive-like behavior in rats through modulation of β-arrestin 2 mediated pathways in hippocampus." Evid Based Complement Alternat Med.2014:902516.  WB ;  Rat.  
      [IF=.32] B?R, Ferda, et al. "New immunohistochemical markers in the differential diagnosis of nonsmall cell lung carcinoma." Turkish Journal of Medical Sciences (2016).  IHC-P ;  Human.  
      研究領域 細胞生物  免疫學  神經生物學  信號轉導  細胞凋亡  激酶和磷酸酶  細胞膜受體  
      抗體來源 Rabbit
      克隆類型 Polyclonal
      交叉反應 Human, Mouse, Rat, 
      產品應用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蠟切片需做抗原修復)
      not yet tested in other applications.
      optimal dilutions/concentrations should be determined by the end user.
      分 子 量 90-92kDa
      細胞定位 細胞漿 細胞膜 
      性    狀 Liquid
      濃    度 1mg/ml
      免 疫 原 KLH conjugated synthetic peptide derived from human Trk B:401-500/822 
      亞    型 IgG
      純化方法 affinity purified by Protein A
      儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
      保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
      PubMed PubMed
      產品介紹 The Trk family of nerve growth factor receptors includes Trk A(also referfed to as Trk A gp140),Trk B and Trk C. The prototype member of this gene family, Trk A, encodes a 140 kDa cell surface receptor, gp140, the expression of which is restricted in vivo to neurons of the sensory spinal and cranial gangliaof neurocrest origin. Nerve growth factor (NGF) stimulates tyrosine phosphorylation of Trk gp 140 in neural cell lines and in embryonic dorsal root ganglia. By comparison, BDNF and to a lesser extent, NT-3, but not NGF, can induce tyrosine phophorylayion of Trk B gp 145. The third member of the Trk receptor family, Trk C incodes a 140 kDa protein, Trk C gp140, that is preferentially expressed in brain tissue and primarily functions as a receptor for NT-3.An additional component of the Trk receptor complex, NGFR p175, binds to neurotrophic factors with low affinity but is required for efficient signaling. NGFR p175 accelerates Trk activation and may recruit downstream dffector molecules to the ligand-bound receptor complex.

      Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.

      Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. Interacts (phosphorylated upon activation by BDNF) with SHC1; mediates SHC1 phosphorylation and activation. Interacts (phosphorylated upon activation by BDNF) with PLCG1 and/or PLCG2; mediates PLCG1 phosphorylation and activation. Interacts with SH2B1 and SH2B2. Interacts with NGFR; may regulate the ligand specificity of the receptor. Interacts (phosphorylated upon ligand-binding) with SH2D1A; regulates NTRK2. Interacts with SQSTM1 and KIDINS220. Interacts (phosphorylated upon ligand-binding) with FRS2; activates the MAPK signaling pathway.

      Subcellular Location:
      Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Note=Internalized to endosomes upon ligand-binding.

      Tissue Specificity:
      facial structures, the submaxillary glands, and dorsal root ganglia. Isoform Isoform TrkB is expressed in the central and peripheral nervous system. In the central nervous system (CNS), expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is mainly expressed in the brain but also detected in other tissues including pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in the brain.

      Post-translational modifications:
      Phosphorylated. Undergoes ligand-mediated autophosphorylation that is required for interaction with SHC1 and PLCG1 and other downstream effectors. Isoform TrkB-T-Shc is not phosphorylated.
      Ubiquitinated. Undergoes polyubiquitination upon activation; regulated by NGFR. Ubiquitination regulates the internalization of the receptor (By similarity).

      Defects in NTRK2 are the cause of obesity hyperphagia and developmental delay (OHPDD) [MIM:613886]. OHPDD is a disorder characterized by early-onset obesity, hyperphagia, and severe developmental delay in motor function, speech, and language.

      Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 2 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 LRRNT domain.
      Contains 1 protein kinase domain.


      Gene ID:

      Database links:

      Entrez Gene: 4915 Human

      Entrez Gene: 18212 Mouse

      Omim: 600456 Human

      SwissProt: Q16620 Human

      SwissProt: P15209 Mouse

      Unigene: 494312 Human

      Unigene: 712776 Human

      Unigene: 130054 Mouse

      Important Note:
      This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

      Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TrkB) Polyclonal Antibody, Unconjugated (bs-0175R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
      Tissue/cell: human nasopharyngeal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
      Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
      Incubation: Anti-Trk-B Polyclonal Antibody, Unconjugated(bs-0175R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
      Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
      Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).
      版權所有 2019-2022 www.fhsyjt.com 北京博奧森生物技術有限公司
      通過國際質量管理體系ISO9001:2008 GB/T19001-2008認證    編號:00115Q211807R1M/1100
      京ICP備05066980號-1         京公網安備110107000727號
      苍井空免费线在线观看,黄在线看片免费人成视频,久久久久人妻一区精品,成年男女免费视频网站不卡,思思久久精品在热线热 <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <文本链> <文本链> <文本链> <文本链> <文本链> <文本链>