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    Rabbit Anti-p75 NGF Receptor antibody (bs-7122R)
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    產品編號 bs-7122R
    英文名稱 p75 NGF Receptor
    中文名稱 神經生長因子受體抗體
    別    名 NGFR; Gp80 LNGFR; ngfrp75; Low affinity nerve growth factor receptor; Low affinity neurotrophin receptor p75NTR; NGF receptor; p75 ICD; CD271; CD271 antigen; Gp80 LNGFR; Low affinity nerve growth factor receptor; Low affinity neurotrophin receptor p75NTR; Nerve growth factor receptor; Nerve growth factor receptor TNFR superfamily member 16; NGF receptor; NGFR; p75 ICD; p75 Neurotrophin receptor; p75 NTR; TNFR Superfamily Member 16; TNFRSF16; Tumor necrosis factor receptor superfamily member 16; TNR16_HUMAN.  
    研究領域 細胞生物  神經生物學  信號轉導  
    抗體來源 Rabbit
    克隆類型 Polyclonal
    交叉反應 Human, Mouse, Rat, Dog, 
    產品應用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復)
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 42kDa
    細胞定位 細胞核 細胞漿 細胞膜 
    性    狀 Liquid
    濃    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human NGFR/p75NTR:151-250/427 <Extracellular>
    亞    型 IgG
    純化方法 affinity purified by Protein A
    儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
    PubMed PubMed
    產品介紹 The low affinity NGFR (Nerve growth factor receptor) is a 75kDa membrane-spanning glycoprotein lacking intrinsic tyrosine kinase activity. p75NGFR interacts with TrkA, the high affinity NGF receptor and potentiates TrkA signaling at low NGF concentrations. The p75 receptor binds nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying specificities. The p75NGFR plays an important role in neurotrophic factor signaling and has been shown to modulate the susceptibility of selective cellular populations to programmed cell death. It is expressed on many neuronal cells types including many embryonic forms and the receptor can be used to isolate neuronal progenitor cells. NGF is important for the development, differentiation and survival of a variety of neuronal and non-neuronal cells. Its action is mediated by binding to two distinct receptors, the high affinity p140 and low affinity p75. p75NGFR binds neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5, and NT-6. p75NGFR belongs to the TNF-R superfamily and is reported to mediate NGF-induced apoptosis.

    Function:
    Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.

    Subunit:
    Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1. Interacts with KIDINS220 and NTRK1. Can form a ternary complex with NTRK1 and KIDINS220 and this complex is affected by the expression levels of KIDINS220. An increase in KIDINS220 expression leads to a decreased association of NGFR and NTRK1. Interacts with LINGO1 (By similarity). Interacts with NTRK2; may regulate the ligand specificity of the NTRK2 receptor. Interacts with NRADD.

    Subcellular Location:
    Membrane; Single-pass type I membrane protein.

    Post-translational modifications:
    N- and O-glycosylated. Phosphorylated on serine residues.

    Similarity:
    Contains 1 death domain. Contains 4 TNFR-Cys repeats.

    SWISS:
    P08138

    Gene ID:
    4804

    Database links:

    Entrez Gene: 4804 Human

    Entrez Gene: 18053 Mouse

    Entrez Gene: 24596 Rat

    Omim: 162010 Human

    SwissProt: P08138 Human

    SwissProt: Q9Z0W1 Mouse

    SwissProt: P07174 Rat

    Unigene: 415768 Human

    Unigene: 681726 Human

    Unigene: 283893 Mouse

    Unigene: 10980 Rat



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

    產品圖片
    Sample: Cerebellum (mouse) Lysate at 40 ug
    Primary: Anti-p75 NGF Receptor (bs-7122R)at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 42kD
    Observed band size: 42kD
    Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p75 NGF Receptor) Polyclonal Antibody, Unconjugated (bs-7122R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p75 NGF Receptor) Polyclonal Antibody, Unconjugated (bs-7122R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-NGFR Polyclonal Antibody, Unconjugated(bs-7122R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    Blank control (blue line): Mouse spleen (fixed with 70% ice-cold methanol overnight at 4℃).
    Primary Antibody (green line): Rabbit Anti- p75 NGF Receptor antibody (bs-7122R),Dilution: 1μg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
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