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      Rabbit Anti-NeuN antibody (bs-10394R)
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      產品編號 bs-10394R
      英文名稱 NeuN
      中文名稱 神經元核抗原抗體
      別    名 FOX3; NeuN; neuronal nuclei antigen; neuronal nuclei; Vertebrate neuron-specific nuclear protein; Neuna60; Neuronal nuclear antigen A60; RNA binding protein fox-1 homolog 3 isoform 1; RNA binding protein, fox 1 homolog (C. elegans) 3; Rbfox3; D11Bwg0517e; Fox-3; Fox3; Hrnbp3; RP23-159O6.5; novel protein; Fox-1 homolog C; fox1 homolog C; FLJ56884; FLJ58356; hexaribonucleotide binding protein 3 RFOX3_HUMAN.  
      Specific References  (3)     |     bs-10394R has been referenced in 3 publications.
      [IF=4.652] Zhang et al. The Neuroprotective Effect of Puerarin in Acute Spinal Cord Injury Rats. (2016) Cell.Physiol.Bioche. 39:1152-64  IHC-P ;  Rat.  
      [IF=2.784] Xiong?LL et al. Tree shrew neural stem cell transplantation promotes functional recovery of tree shrews with a hemi?sectioned spinal cord injury by upregulating nerve growth factor expression. Int J Mol Med. 2018 Jun;41(6):3267-3277.  IF ;  tree shrews.  
      [IF=3.076] Tang Q et al. Ferroptosis is newly characterized form of neuronal cell death in response to arsenite exposure.Neurotoxicology. 2018 Jul;67:27-36.   IF ;  Mouse.  
      研究領域 細胞生物  神經生物學  細胞凋亡  轉運蛋白  細胞類型標志物  表觀遺傳學  
      抗體來源 Rabbit
      克隆類型 Polyclonal
      交叉反應 Human, Mouse, Rat, Horse, Sheep, Bee, 
      產品應用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/test ICC=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復)
      not yet tested in other applications.
      optimal dilutions/concentrations should be determined by the end user.
      分 子 量 34kDa
      細胞定位 細胞核 細胞漿 
      性    狀 Liquid
      濃    度 1mg/ml
      免 疫 原 KLH conjugated synthetic peptide derived from human FOX3:101-200/312 
      亞    型 IgG
      純化方法 affinity purified by Protein A
      儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
      保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
      PubMed PubMed
      產品介紹 The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. [provided by RefSeq, Jul 2008]

      Function:
      RNA-binding protein that regulates alternative splicing events.

      Subcellular Location:
      Nucleus. Cytoplasm.

      Tissue Specificity:
      Expressed in activated, but not resting, CD4+ T-cells and activated monocytes.

      Similarity:
      Contains 1 RRM (RNA recognition motif) domain.

      SWISS:
      A6NFN3

      Gene ID:
      146713

      Database links:

      Entrez Gene: 146713 Human

      Entrez Gene: 52897 Mouse

      Entrez Gene: 287847 Rat

      SwissProt: A6NFN3 Human

      SwissProt: Q8BIF2 Mouse

      Unigene: 135229 Human

      Unigene: 341103 Mouse

      Unigene: 143966 Rat



      Important Note:
      This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

      產品圖片
      Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
      Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
      Incubation: Anti-NeuN/FOX3 Polyclonal Antibody, Unconjugated(bs-10394R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
      Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
      Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
      Incubation: Anti-NeuN/FOX3 Polyclonal Antibody, Unconjugated(bs-10394R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
      Blank control:A549.
      Primary Antibody (green line): Rabbit Anti-NeuN antibody (bs-10394R)
      Dilution: 1μg /10^6 cells;
      Isotype Control Antibody (orange line): Rabbit IgG .
      Secondary Antibody : Goat anti-rabbit IgG-PE
      Dilution: 1μg /test.
      Protocol
      The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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