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          Mouse Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid antibody (bsm-41414M)
          說明書: 下載

          產品編號 bsm-41414M
          英文名稱 SARS-CoV-2 (2019-nCoV) Nucleocapsid
          中文名稱 SARS冠狀病毒2核衣殼蛋白單克隆抗體
          別    名 SARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 NP; nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2]; novel coronavirus N Protein; novel coronavirus Nucleocapsid Protein; 2019-nCoV Nucleoprotein; 2019-nCoV N; 2019nCoV N; 2019-nCoV N Protein; 2019 ncov N Protein; 2019-nCoV nucleocapsid protein.  
          抗體來源 Mouse
          克隆類型 Monoclonal
          克 隆 號 7E1B
          交叉反應 SARS-CoV-2
          產品應用 WB=1:500-2000 ELISA=1:5000-10000 
          not yet tested in other applications.
          optimal dilutions/concentrations should be determined by the end user.
          分 子 量 46kDa
          性    狀 Liquid
          濃    度 ≥1 mg/ml
          免 疫 原 Recombinant SARS-CoV-2 Nucleocapsid Protein:full length  
          亞    型 IgG2b
          純化方法 affinity purified by Protein G
          儲 存 液 0.01M PBS(pH7.4) with 0.1% Proclin300
          保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
          PubMed PubMed
          產品介紹 Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

          Important Note:
          This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

          Lane 1: SARS-CoV-2 N protein(Cat# bs-41408P) at 50ng
          Lane 2: SARS-CoV-2 N protein(Cat# bs-41408P) at 25ng
          Lane 3: SARS-CoV-2 N protein(Cat# bs-41408P) at 5ng
          Lane 4: SARS-CoV-2 N protein(Cat# bs-41408P) at 2ng
          Lane 5: SARS-CoV-2 N protein(Cat# bs-41408P) at 1ng
          Lane 6:SARS-CoV-2 N protein(Cat# bs-41408P) at 0.5ng
          Primary: Anti- SARS-CoV-2 N protein (bs-41414M) at 1/1000 dilution
          Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
          Predicted band size: 46 kD
          Observed band size: 46 kD
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